primary antibodies against jak2  (Bioss)

Journal: Cancers

Article Title: Melatonin Modulates the SIRT1-Related Pathways via Transdermal Cryopass-Laser Administration in Prostate Tumor Xenograft

doi: 10.3390/cancers15204908

Figure Lengend Snippet: Representative photomicrographs of xenograft PGC-1α immunostainings of vehicle cryopass-laser-treated mice ( A ) and melatonin cryopass-laser-treated mice ( B ). Representative photomicrographs of the negative controls of PGC-1α immunofluorescence, ( D )—vehicle cryopass-laser-treated group. Bars: 20 μm. The white arrows indicate the mitochondrial PGC-1α positivity. The graph ( C ) summarizes PGC-1α immunomorphometrical measurements. * p ≤ 0.05 vs. melatonin cryopass-laser group. PGC-1α: PPAR-gamma coactivator 1-alpha.

Article Snippet: The xenograft sections were incubated for 1 h at 37 °C and for 1 h at room temperature with the following primary antibodies: rabbit polyclonal antibody against anti-Ki67 (diluted 1:100; Abcam, Cambridge, UK); anti-CD31; goat polyclonal antibody against MMP2 (diluted 1:200; Santa Cruz Biotechnology, Dallas, TX, USA); rabbit polyclonal antibody against SIRT1 (diluted 1:250; Abcam, Cambridge, UK); rabbit polyclonal antibody against PGC-1α (diluted 1:400; Abcam, Cambridge, UK); rabbit polyclonal PPAR γ (diluted 1:500; Abcam, Cambridge, UK; and rabbit polyclonal against NF-kB (diluted 1:350; Abcam, Cambridge, UK).

Techniques: Immunofluorescence

Journal: Cancers

Article Title: Melatonin Modulates the SIRT1-Related Pathways via Transdermal Cryopass-Laser Administration in Prostate Tumor Xenograft

doi: 10.3390/cancers15204908

Figure Lengend Snippet: Representative photomicrographs of xenograft sirtuin1 immunostaining of vehicle cryopass-laser-treated mice ( A ) and melatonin cryopass-laser-treated mice ( B )—Bars: 8 μm. Representative photomicrographs of immunofluorescence negative control-vehicle cryopass-laser-treated group ( D ). Bar: 20 μm. The white asterisks indicate the cytoplasmatic sirtuin1 positivity and the white circle indicates a representative sirtuin1 positive tumor cell nucleus. Graph ( C ) summarizes the sirtuin1 immunomorphometrical measurement. * p ≤ 0.05 vs. melatonin cryopass-laser group. SIRT1: sirtuin1.

Article Snippet: The xenograft sections were incubated for 1 h at 37 °C and for 1 h at room temperature with the following primary antibodies: rabbit polyclonal antibody against anti-Ki67 (diluted 1:100; Abcam, Cambridge, UK); anti-CD31; goat polyclonal antibody against MMP2 (diluted 1:200; Santa Cruz Biotechnology, Dallas, TX, USA); rabbit polyclonal antibody against SIRT1 (diluted 1:250; Abcam, Cambridge, UK); rabbit polyclonal antibody against PGC-1α (diluted 1:400; Abcam, Cambridge, UK); rabbit polyclonal PPAR γ (diluted 1:500; Abcam, Cambridge, UK; and rabbit polyclonal against NF-kB (diluted 1:350; Abcam, Cambridge, UK).

Techniques: Immunostaining, Immunofluorescence, Negative Control

Journal: Aging (Albany NY)

Article Title: The oncogenic miR-429 promotes triple-negative breast cancer progression by degrading DLC1

doi: 10.18632/aging.205051

Figure Lengend Snippet: Primer sequences for qRT-PCR.

Article Snippet: Overnight incubation of the rabbit polyclonal antibody against DLC1 (ab126257, 1:1000, Abcam, UK) and GAPDH (1:5000, Proteintech, China) with the membrane was executed at 4° C. These were subsequently rinsed thrice with TBST (Tris-buffered saline with Tween 20) for about 15 min each.

Techniques: Sequencing

Journal: Aging (Albany NY)

Article Title: The oncogenic miR-429 promotes triple-negative breast cancer progression by degrading DLC1

doi: 10.18632/aging.205051

Figure Lengend Snippet: miR-429 targets and negatively regulates the expression of DLC1. ( A ) Volcano map of DEmRNAs in TCGA-TNBC data. The upregulated genes with differential expression were in Blue and the downregulated ones were in Red; ( B ) Venn diagram of potential target genes and downregulated DEmRNAs for miR-429 in 3 databases; ( C ) Pearson correlation analysis of miR-429 and nine overlapping genes; ( D ) Relative expression of DLC1 in human normal breast cell (MCF- 10A) and TNBC cell line (MDA-MB-468) was detected by Western Blot; ( E ) The binding site sequence of DLC1 3’UTR to miR-429 was predicted by TargetScan and validated by a dual luciferase assay; ( F ) qRT-PCR and Western blot ( G ) analysis of DLC1 expression in TNBC cell transfected with miR-429 mimic, mimic NC, miR-429 Inhibitor or Inhibitor NC. All figures are at a scale of 50μm. (* p < 0.05, ** p < 0.01, and *** p < 0.001).

Article Snippet: Overnight incubation of the rabbit polyclonal antibody against DLC1 (ab126257, 1:1000, Abcam, UK) and GAPDH (1:5000, Proteintech, China) with the membrane was executed at 4° C. These were subsequently rinsed thrice with TBST (Tris-buffered saline with Tween 20) for about 15 min each.

Techniques: Expressing, Quantitative Proteomics, Western Blot, Binding Assay, Sequencing, Luciferase, Quantitative RT-PCR, Transfection

Journal: Aging (Albany NY)

Article Title: The oncogenic miR-429 promotes triple-negative breast cancer progression by degrading DLC1

doi: 10.18632/aging.205051

Figure Lengend Snippet: miR-429 increases TNBC cell proliferation, migration, and invasion through degradation of DLC1. ( A ) Protein levels of DLC1 relative to GADPH in cell assessed by Western Blot after transfection with indicated vectors, i.e., siRNA, miRNA, or inhibitor; ( B ) The cell proliferation was assessed by CCK-8 after transfection (0h, 24h, 48h,72h); ( C ) Wound healing assays were used to detect migration of cells after transfection (magnification, × 100). ( D ) The cell invasion was determined by transwell after transfection (magnification, × 200). All figures were 50 μm of scale bar. (* p < 0.05, ** p < 0.01, and *** p < 0.001).

Article Snippet: Overnight incubation of the rabbit polyclonal antibody against DLC1 (ab126257, 1:1000, Abcam, UK) and GAPDH (1:5000, Proteintech, China) with the membrane was executed at 4° C. These were subsequently rinsed thrice with TBST (Tris-buffered saline with Tween 20) for about 15 min each.

Techniques: Migration, Western Blot, Transfection, CCK-8 Assay

Journal: Aging (Albany NY)

Article Title: The oncogenic miR-429 promotes triple-negative breast cancer progression by degrading DLC1

doi: 10.18632/aging.205051

Figure Lengend Snippet: Correlation between DLC1 expression and clinicopathological features in 114 TNBC patients.

Article Snippet: Overnight incubation of the rabbit polyclonal antibody against DLC1 (ab126257, 1:1000, Abcam, UK) and GAPDH (1:5000, Proteintech, China) with the membrane was executed at 4° C. These were subsequently rinsed thrice with TBST (Tris-buffered saline with Tween 20) for about 15 min each.

Techniques: Expressing

Journal: Aging (Albany NY)

Article Title: The oncogenic miR-429 promotes triple-negative breast cancer progression by degrading DLC1

doi: 10.18632/aging.205051

Figure Lengend Snippet: DLC1 expression correlates with the anticancer properties of TNBC. ( A ) HE and IHC staining of TNBC. Scale bar = 100 μm. ( B ) Correlation of DLC1 immunostaining intensity with clinical TNM stage and histopathological grade of TNBC, respectively. ( C ) Kaplan-Meier survival curves for overall survival of 114 patients with TNBC according to the DLC1 expression. Patients were stratified into high-expression and low-expression groups by median expression. (* p < 0.05, ** p < 0.01, and *** p < 0.001).

Article Snippet: Overnight incubation of the rabbit polyclonal antibody against DLC1 (ab126257, 1:1000, Abcam, UK) and GAPDH (1:5000, Proteintech, China) with the membrane was executed at 4° C. These were subsequently rinsed thrice with TBST (Tris-buffered saline with Tween 20) for about 15 min each.

Techniques: Expressing, Immunohistochemistry, Immunostaining